Anaerobs

General
- Should be grown in a special incubator.
- 5 days
- remember that aerob bacteria can also grow in this media, so also culture in aerob environment for comparison.
- mostly for abces, and positive blood culture.

Division
- Gram negative bacil
   - before the MALDI era, it was differentiated from gram positive bacil (GPB) by susceptibility  to collistin and resistant to vancomycin (GPB is vice versa)
   - most important clinically
   - Bacteriodes fragilis (kanamycin and oxacillin resistant)
   - Prevotella (black)
   - Poryphyromonas
   - Fusobacterium nucleatum (looks like needles on microscope )

- Gram positive bacil
   - Before MALDI era, the species are differentiate using batteries of chemical reaction, since they release volatile and non volatile acid from glucose fermentation.
   - Propioni (significant in the case of prosthesis, on microscope look like yellow pearle).
   - Bifidobacterium
   - Actinomycetes
   - Lactobacillus (can also grow in oxygen milieu).
    - Clostridium (produce iso-caproic acid, see previous item on C. difficile).

- Gram positive/ negative cocci
   - Clinically not important.
   - Veilonella