Trivia Microbiology

Antibiogram
- Intermediair in EUCAST 'means' higher dose might kills the bacteria. In CLSI, it means preferably not use the antibiotics.
- Longer incubation needed for bacteriostatic antibiotic, for example: clindamycin.

Antibiotic
- Against anaerobes
Antibiotics active against anaerobes: penicilines, first generation cephalosporines, clindamycin and metronidazoles.

- Quinolones
   - Against Gram positive: moxifloxacine > levofloxacine > ciprofloxacine
   - Against Gram negative, especially Pseudomonas: ciprofloxacine > levofloxacine > moxifloxacine
   - Active against anaerob: moxifloxacine.

Treatment
Febrile neutropenia:
- cefepime + amikacin can be given.

Meningitis and brain abces
- There are limited publications of treatment of Acinetobacter meningitis. in EUCAST there is no information cephalosporines susceptibility. Meropenem is one of the possibility.
- Rx/ of brain abses: ceftriaxon, metronidazol, vancomycin (to cover S.aureus, or Strep after fracture/ operation on the head area).

Pneumonia
- In hospital acquired pneumonia: pip/taz  -->  moxifloxacin (good activity against S. pneumonia, this drug is not good against Pseudomonas)
- In health care associated pneumonia: ceftriaxone
- In community acquired pneumonia: ampicilline --> moxifloxacine

Prostatitis
- Rx/ Bactrim or Ciprofloxacin.

UWI
- Temocillin, 2g 2dd iv. as alternative in treating UWI in case of ESBL and ciprofloxacin resistance.
- Cefuroxim covers a lot of Gram + but also for Gram -: E. coli, Klebsiella and Proteus.

Listeria
- In baby: almost always from mother.
- Mother gets it from food.

Premature rupture of membrane
- Erythromycin (iv) followed by Azithromycin (po)

Skin and soft tisssue infection
- Streptococcus angionosus can give abses, but is almost always susceptible to penicillin.

Toxoplasmose
- Infection in the first trimester: Treatment with spiramycin and ampicillin for mother.

Echinocandin
- Does not accumulated in the urine, so not good for the treatment of fungal in the urine. Then use Fluconazol.
- Fungal infection is mainly hematogenous.




Antibiotics and laboratory
Disk diffusion test
- 15 minutes on room temperature maximum. Reason: antibiotic will have otherwise a quicker start than bacteria. The results will overestimate the antibiotic effect.
- Corkscrew effect (figure): amoxicillin in the middle and third generation cephalosporins and aztreonam around it. This is done to visualize ESBL bacterias.





Don't forget
- Never give aminoglycosides (gentamycin, amikacin) alone.
- Don't give third generation antibiotics to ESCCAPM organisms that can produce cAMP.
- Clindamycin is good agains toxin producing bacteria (for example: GAS).

- Bordetella parapertussis does not grow on agar which has blood content.

- Aerogenes ureaticum often in kidney stone.

- Streptococcus antibiogram not in Muller Hinton but in blood agar.

Recognition of bacteria
Colony:
- Corynebacterium: dry, rugged
- Klebsiella: mucoid
- E. coli: yellow, not mucoid in general on CLED
- Pseudomonas: not mucoid in general, only mucoid in patients with cystic fibrosis.
- Enterococcus: grey, white inside, not dominatn
- Lactobacillus: cloud figure
- Yeast: flat not white top
- In general size: Streptococcus < Enteroccus < Staphylococcus
- Streptococcus angionosus: caramel smell
- Staphylococcus: catalase positive, white,

Gram:
- Corynebacterium: baseball bat, Chinese letters


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