Dermatophyte
- Isolated from hair, nails
- Tricophyton, Microsporidia
- Macroscopic; flat, hairy, white to yellow
- Grow mainly in dermatohoyte specific medium (Sabouraud with added antibiotics and cyclohexmide to inhibit saprotrophic growth)
- Yeast more in nails of the finger, Aspegillus more in the nails of the toes
Yeast vs. mould
- Macroscopic: yeasts have shiny surface (look like m&ms candies)
- Yeast can grow within 1-3 days, moulds longer
Microscopic recognition
- Micro or macroconidia (conidia means spores)
- Mycellum is where the conidia hangs (spores can be free)
- Septae is the division of mycellum
Organizational stuff
- The specimens are placed in Sabouraud and dermatophyte specific medium
- When shiny surface colonies are seen: probably yeast
- When mould is suspected in dermatophyte specific medium --> reisolation in diluted Sabouraud, so spores are formed by the fungi (spores are formed in barren condition)
- When mould is suspected in usual Sabouraud --> reisolation in diluted Sabouraud and in dermatophyte specific medium
- Isolated from hair, nails
- Tricophyton, Microsporidia
- Macroscopic; flat, hairy, white to yellow
- Grow mainly in dermatohoyte specific medium (Sabouraud with added antibiotics and cyclohexmide to inhibit saprotrophic growth)
- Yeast more in nails of the finger, Aspegillus more in the nails of the toes
Yeast vs. mould
- Macroscopic: yeasts have shiny surface (look like m&ms candies)
- Yeast can grow within 1-3 days, moulds longer
Microscopic recognition
- Micro or macroconidia (conidia means spores)
- Mycellum is where the conidia hangs (spores can be free)
- Septae is the division of mycellum
Organizational stuff
- The specimens are placed in Sabouraud and dermatophyte specific medium
- When shiny surface colonies are seen: probably yeast
- When mould is suspected in dermatophyte specific medium --> reisolation in diluted Sabouraud, so spores are formed by the fungi (spores are formed in barren condition)
- When mould is suspected in usual Sabouraud --> reisolation in diluted Sabouraud and in dermatophyte specific medium
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